Matrix Metalloproteinase Collagenase/Gelatinase A by a Recombinant Membrane-Type 1 Processing of a Precursor of 72-Kilodalton Type IV
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چکیده
Membrane-type 1 matrIx metalloproteinasethat is associatedwith the proteolytic activation of progelatlnase A was expressed as a recombinant fusion protein in Escherichia coiL The recombinant enzyme cleaved the propeptide sequence of gelatinase A in a sequence-specific manner. A mutant progelatinase A that has a substitution ofAsnss@Leu to ile-Val was not processed at all. The processing was blocked by tissue inhibitor of metalloproteinases-2 or BB-94 but not by tissue inhibitor of metallopro teinases-1. Thus, membrane-type 1 matrix metalloproteinase is a direct activator of progelatinase A without requiring additional proteases.
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تاریخ انتشار 2006